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Abstract Dissecting the relationship between gene function and substitution rates is key to understanding genome-wide patterns of molecular evolution. Biochemical pathways provide powerful systems for investigating this relationship because the functional role of each gene is often well characterized. Here, we investigate the evolution of the flavonoid pigment pathway in the colorful Petunieae clade of the tomato family (Solanaceae). This pathway is broadly conserved in plants, both in terms of its structural elements and its MYB, basic helix–loop–helix, and WD40 transcriptional regulators, and its function has been extensively studied, particularly in model species of petunia. We built a phylotranscriptomic data set for 69 species of Petunieae to infer patterns of molecular evolution across pathway genes and across lineages. We found that transcription factors exhibit faster rates of molecular evolution (dN/dS) than their targets, with the highly specialized MYB genes evolving fastest. Using the largest comparative data set to date, we recovered little support for the hypothesis that upstream enzymes evolve slower than those occupying more downstream positions, although expression levels do predict molecular evolutionary rates. Although shifts in floral pigmentation were only weakly related to changes affecting coding regions, we found a strong relationship with the presence/absence patterns of MYB transcripts. Intensely pigmented species express all three main MYB anthocyanin activators in petals, whereas pale or white species express few or none. Our findings reinforce the notion that pathway regulators have a dynamic history, involving higher rates of molecular evolution than structural components, along with frequent changes in expression during color transitions. 

In any research computing (RC) environment, the role of the user support group is a combination of education, consulting, and outreach. As this role is the most public-facing of a research computing group's team, it is important to ensure an excellent level of support is provided to users. The Research Computing group at the University of Colorado (CURC) Boulder maintains a large-scale computing cluster with several hundred active users, among other services, and has done so since 2011. The user support group at CURC provides a variety of services intended to support these users. This paper describes those services the CURC group provides, as well as explores the various ways that these services have been improved in 2018-19. The impact on users is also assessed. 

Advances in the volume, diversity, and complexity of research data and associated workflows requires enhanced capabilities to access, secure, reuse, process, analyze, understand, curate, share, and preserve data. To address this need at the regional level the University of Colorado, Colorado State University, and the University of Utah formed a "Cyberteam" in 2017 to provide cyberinfrastructure (CI) support to researchers at institutions in the Rocky Mountain Advanced Computing Consortium (RMACC) encompassing states across the Intermountain West. The Cyberteam is comprised of CI professionals across the three institutions who collaborate closely, sharing expertise and resources. Since its establishment, the Cyberteam has worked to broaden accessibility and options for computing, storage, and data publishing for RMACC researchers; enhance training on data- and workflow-oriented topics; improve engagement with researchers using CI; and better understand user needs and challenges. One key accomplishment has been the development of a series of focus group and survey instruments to achieve better understanding the CI needs and challenges of researchers across a diverse spectrum of disciplines. This paper provides an overview of the RMACC Cyberteam's objectives, accomplishments, challenges, and future direction.